Afghan Turkestan, also known as Southern Turkestan, is a region in northern Afghanistan, on the border with the former Soviet republics of Turkmenistan, Uzbekistan and Tajikistan. In the 19th century, there was a province in Afghanistan named Turkestan with Mazari Sharif as provincial capital. The province incorporated the territories of the present-day provinces of Balkh, Kunduz, Jowzjan, Sar-e Pol, and Faryab. In 1890, Qataghan-Badakhshan Province was separated from Turkestan Province. It was later abolished by Emir Abdur Rahman.

The whole territory of Afghan Turkestan, from the junction of the Kokcha river with the Amu Darya on the north-east to the province of Herat on the south-west, was some 800 kilometres (500 mi) in length, with an average width from the Russian frontier to the Hindu Kush of 183 km (114 mi). It thus comprised about 150,000 km² (57,000 sq mi) or roughly two-ninths of the former Kingdom of Afghanistan.